3D-Printable Model: Stomach-Vagus Electrophysiology Testing Chamber with Mechanical Stimulus Placement Grid v1.0.0
Internal Dataset

UID: 22

Author(s): Horn, Charles C.*; Sciullo, Michael; Farr, Laura * Corresponding Author + University of Pittsburgh Author

Description
This data package contains 3D printing files (in multiple formats) and instructions for producing a testing chamber.

The gastric vagus supplies critical information to the brain for the control of feeding and aversive signaling, such as nausea and vomiting. This perfusion chamber model (with a grid for testing mechanical stimuli) is designed to test mechanical or chemical stimuli applied to the stomach while recording electrophysiological responses from the vagus, from teased fiber bundles or the whole nerve. The dissected stomach, with attached vagus, of small laboratory animals, including musk shrew (a vomiting species) and mouse (a non-vomiting species), can be placed in the perfusion (stomach) compartment and kept alive with oxygenated Krebs solution flowing through the input port and exiting through the two outflow ports (2 are used to ensure that the compartment will not overflow). The included detachable grid allows the experimenter to record the placement of a mechanical probe in alphanumeric coordinates; Von Frey filaments (e.g., 10 mg force) can to used to apply mechanical stimulation. The recording compartment contains holes for placement of fine wire electrodes, which can be guided to the top of the model for connections to recording equipment (e.g., high impedance head stage and amplifier system). The two compartments are sealed by running the nerve connected to the stomach under a plastic gate, coated with vacuum grease; the gate is lowered into grooves that are part of the model. Based on this model, it is possible to produce a testing chamber for a larger species, such as the laboratory rat, by extending the size of the stomach compartment.

Keywords
Access via NIH 3D Print Exchange

Two models in multiple formats, full description, and printing and assembly instructions.
Accession #: 3DPX-003134

Access Restrictions
Free to all
Access Instructions
3D printing files and instructions for printing and assembly available for free download. Licensed under CC BY-SA.
Data Type
Electrophysiological
Software Used
Blender
Dataset Format(s)
X3D (.x3d), STL (.stl), BLEND (.blend), IGS (.igs)
Dataset Size
501 KB
Data Catalog Record Updated
2018-11-05

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